Custom Antibody

Ⅰ. Service

Commercially available plant protein antibodies are limited in number and variety. Aside from commonly used antibodies (e.g., tags, internal references) and antibodies for the model plant Arabidopsis thaliana, most plant proteins lack readily available antibodies and thus require customized solutions. To address researchers’ diverse and in-depth needs for antibodies, Solarbio offers comprehensive, one-stop services—from plant gene to research antibody preparation and subsequent validation. This service is backed by an advanced R&D platform, a professional development team, and instruments from internationally renowned brands—ensuring reliable customization and testing of plant antibodies.

Ⅱ. Service Requirements

The client should provide the gene/protein ID, nucleotide/amino acid sequence, and species information for the plant protein antigen.

Please specify the antigen type (whether a designed peptide or an expressed recombinant protein), who will prepare the antigen, and the type of antibody required (polyclonal or monoclonal).

Ⅲ. Pricing & Deliverables

Kindly check the details of CRO-Preparation of Mouse Polyclonal Antibody, CRP-Preparation of Mouse Monoclonal Antibody and CRO-Polyclonal Antibody Production Services.

1. Service Overview

Beijing Solarbio Science & Technology Co., Ltd., established in 2004, is now introducing a mouse polyclonal antibody production service based on over a decade of expertise and experience. The entire process can be completed within just 3–4 weeks!

1. Service

• Immunize five Balb/c mice, perform ELISA to assess serum titers, and once the antiserum titer meets the required standard, collect whole blood and isolate the antiserum.

Price: USD 800~1000 per project.

III. Service Advantages

• Short Turnaround: Obtain high-quality antisera in only 3–4 weeks, saving time.
• Low Antigen Requirement: Immunizing five mice requires only 300–500 µg of antigen, conserving your valuable materials.
• Simple Protocol: No emulsification needed; use intramuscular injection and only two injections in total.
• Adjuvant: Water-based adjuvant that preserves the antigen’s spatial conformation, making it easier to generate antibodies recognizing conformational epitopes. It is non-toxic and contains no protein components or additional immunogens.
• Mouse Immunization Benefits: More inbred strains ensure purer sera and higher-quality antibodies.
• Sufficient Serum Volume: 1 ml of high-quality antiserum typically meets most experimental needs, especially if only a few confirmatory assays are needed.

1. Deliverables

• 1 ml of tested mouse antiserum
• Experimental report

Ⅰ. Service

1. Antigen preparation (inquiry);
2. Immunize Balb/C mice;
3. Mouse antiserum detection (WB/ELISA detection of endogenous protein);
4. If the test results are qualified, cell fusion, cell line screening, subcloning, and establishment of cell lines that stably secrete antibodies will be carried out;
5. Identification of subtypes of antibody heavy chain (Fcγ) secreted by cell lines;
6. Expanded production of antibodies, preparation of ascites fluid, and purification of Protein A/G.

Ⅱ. Submit results to clients

1. Experiment report;
2. Cell lines and antibodies.
3. Service description

Ⅲ. Note

1. Peptide synthesis requires additional charges;
2. Special requirements will be negotiated separately;
3. Specially modified monoclonal antibodies such as phosphorylation, methylation, and acetylation will be negotiated separately;
4. Finally, 10mg of antibody will be provided.

The following contents are all for rabbit polyclonal antibody preparation services. For customers who need to immunize rats, goats or other animals, please inquire.

1. Service

1) Immunize two rabbits per antigen, with four rounds of immunization.

2) Once the antiserum meets the required titer standards, collect whole blood and separate the antiserum.

3) Purify antibodies via affinity chromatography.

4) Determine the titer of purified antibodies by ELISA (antibody titer).

1. Antigen Preparation (inquiry)

• Peptides: 10 mg total (5 mg for immunization, 1 mg for detection/assays, 4 mg for affinity column preparation), purity >90%.
• Recombinant Proteins: Protein solution at 2 mg/mL, purity >85%, total quantity >5 mg.

III. Service Items & Pricing Standards (USD)

1. Note

1.Peptides synthesized by Solarbio: quantity 10 mg, purity >90%, peptide length 10–18 amino acids.

2.Polyclonal antibody production for modified peptide antigens requires a separate quote.

3.Solarbio can also validate the antibody in other assays (WB, IHC) using samples provided by the customer; service fees are charged according to our technical service pricing.

4.Free salt precipitation (ammonium sulfate precipitation) is available. The choice of affinity purification method is up to the customer.