Ⅰ. Service
1) Gene Synthesis
2) Vector Construction
3) Prokaryotic Expression
4) SDS-PAGE Analysis of Protein Expression
5) Protein Purification
Ⅱ. Vector Construction & Prokaryotic Expression
Steps
|
Price(USD)
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Period
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Primer Design
|
Free
|
2days
|
Gene Synthesis
|
0.2/bp (≤3500bp); 0.4/bp (3501~6000bp)
|
2-4 weeks
|
Vector Construction & Subcloning
|
150/gene
|
1-2 weeks
|
Protein Expression Testing
|
100/protein
|
1-2 weeks
|
Expression & Purification
|
334/protein
|
1-2 weeks
|
• For purified protein yields exceeding 3 mg, an additional fee of USD200 per 3 mg will be charged.
• By default, the purified protein is with His-tag. If you require tag removal, the cost is USD168 per 3 mg, with other amounts subject to quotation.
|
Ⅲ. Materials Provided by the Client
1) Protein or Gene Sequence
2) Template Materials for PCR Amplification (plasmids, cells, tissues, etc.), ensuring no biosafety concerns
- Deliverables
1) Experimental Report
2) Purified Recombinant Protein